Figure 7
From: Glucocorticoid therapy regulates podocyte motility by inhibition of Rac1
Effect of Rac1 inhibition on podocyte barrier function. The effects of Rac1 inhibition with the small molecule EHT 1864 on electrical resistance across a monolayer of podocytes was examined using electrical cell-substrate impedance sensing (ECIS). Here, electrical resistance is used as a surrogate marker for cell barrier integrity, with higher electrical resistance implying higher barrier integrity. (a) Shows the combined results of three experiments normalised to the vehicle control. Each experiment consisted of 3 plated wells of cells per condition, with each experiments lasting for 60 hours following treatment. The experiment was performed three times. Measured electrical resistances during the final 30 minutes of the experiment for each condition were used for final statistical analysis. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test. **** = p value < 0.0001. The thin grey lines represent standard error of the mean. (b) shows an example of a single experiment, with the resistance on the y-axis normalised to measured resistance at the time of treatment for each condition.
