Figure 1
Uremia does not affect neointima formation in carotid arteries after vascular injury in C57BL/6 mice. (A) Study outline: C57BL/6 mice underwent 5/6 nephrectomy (NX) or sham operations in two steps (timepoints NX1 and NX2), and subsequently carotid wire injury and magnetic resonance imaging (MRI). (B) Quantification of cross-sectional vascular wall area by in vivo MRI (average of 4 cross-sections/mouse; n = 5 NX and n = 4 sham) and histology (average of 8–12 sections/mouse; n = 11 NX and n = 8 sham) in injured carotids of NX and sham C57BL/6 mice 6 weeks after wire injury. (C) Quantification of carotid wall area by histology. Three anatomical locations, series 1–3 (S1–S3, 2–4 sections/mouse/per series, n = 11 NX and n = 8 sham), were quantified. (D) Representative pictures of Trichrome stained injured and control carotid arteries from NX and sham mice. Scale bar, 200 μm. (E) Quantification of cross-sectional intimal and (F) medial area in injured and control carotid arteries from NX and sham mice (average of 8–12 sections/mouse, n = 11 NX and n = 8 sham). Data are presented as mean ± SEM. Unpaired t-test (B) with correction for multiple comparisons (C), 1-way ANOVA followed by Sidak’s multible comparison test (E,F). *P < 0.05.
