Figure 2
From: Apoptosis inhibitor of macrophage ameliorates fungus-induced peritoneal injury model in mice
Expression of surface markers of inflammatory cells in the zymosan-induced peritonitis model. Immunostaining of the peritoneal wall for iNOS (a,b), CD206 (c,d) and Ly6B2 (e,f). On day 28, the number of iNOS positive macrophages with M1 polarity ((a) red color) and Ly6B2 positive cells ((e) red color) was significantly higher in AIM −/− mice than AIM +/+ mice, while CD206-positive macrophage expression with M2 polarity ((c) green color) was higher in AIM +/+ mice compared with AIM −/− mice. The nuclei were counterstained with 4′, 6-diamidino-2-phenylindole (DAPI, blue color). The second and fourth rows show higher magnifications of the images in the boxed areas of the first and third rows, respectively. Positive areas were assessed by morphometry (b,d and f). W: AIM +/+ mice; K: AIM −/− mice; CTL: control normal AIM +/+ mice, Scale bars, 100 μm. n = 6 for each group.
