Figure 2
Sirt1 depletion increases IgE/Ag-induced mast cell activation. BMMCs treated with Sirt1 or control (mock) siRNA (a–d) and BMMCs from Sirt1 −/− (KO) or WT mice (e–h) were stimulated with IgE/Ag in the presence or absence of REV. Acetylation or phosphorylation of signaling molecules (a,e) and releases of β-Hex (b,f), LTC4 (c,g) and PGD2 (d,h) were evaluated. The immunoblot data (a,e) are representative of three independent experiments, and the values (b–d, f–h) indicate the means ± S.E.M. from three independent experiments with different BMMCs (*P < 0.05, **P < 0.01 and ***P < 0.001 vs. mock or WT in each treatment; ##P < 0.01 and ###P < 0.001 vs. DNP-HSA alone in each group).
