Figure 1

Differential sites and regions identified in the 450 K array analyses. (a) Volcano plot depicting differences in DNAm levels between controls and patient (T1) for each probe in the corrected 450 K dataset (indicated on X axis) against FDR (indicated on Y axis, on –log10 scale). Dashed horizontal line denotes FDR threshold of 0.1 while dashed vertical lines denote DNAm difference thresholds of −0.05 and 0.05, respectively. (b) Differential DNAm detected by DMRcate in the promoter region of the SRPK3 gene (chrX:153, 046, 386–153, 046, 482). (c) Volcano plot depicting differences in DNAm levels between patients (T1) and patients (T2) as described in panel (a). (d) DNAm levels of seven sites which show reversion of DNAm post-treatment. ***Indicate an FDR < 0.001.