Figure 3
Validation and replication of top-ranking hits by pyrosequencing. (a) Boxplots showing differences in DNAm levels of discovery cohort T-cell samples as measured by pyrosequencing (FDR < 0.01, Student’s t-test). (b) Two top-ranked hits (cg07280807 and cg18752527) were verified as being differentially methylated in T-cell samples of the replication cohort (FDR < 0.05, one-sided t-test). (c) Verification of differential methylation of cg18752527 (HECW2) in the discovery (left) and the replication cohort (right) in DNA derived from whole blood (FDR < 0.05, two-sided t-test). (d) Verification of cg16529483 and cg24496423 (SRPK3) differential methylation in the discovery cohort in DNA derived from whole blood (FDR < 0.01, two-sided t-test).
