Figure 6
The expression of sclerostin, β-catenin and RANKL of peri-implant bone during remodeling after impact. (a) Immunofluorescence staining on sclerostin in trabeculae at different time points after impact. White arrow indicates the expression of sclerostin. (b) Extracted total RNA in agarose gel electrophoresis. In this figure, 3 bands representing 28 s, 18 s and 5 s rRNA are showed clearly, which demonstrates that the extracted total RNA was not degraded. (c) The SOST PCR amplification curve. The marker in this figure represents the threshold of SOST in PCR amplification. (d) The fluorescence intensity of sclerostin immunofluorescence staining. (e) The expression of SOST, β-catenin and RANKL mRNA (7d and 14d n = 5, 28d n = 6, control n = 8). Values are expressed as means ± SD. *P < 0.05 vs. the control group, #P < 0.05 vs. 7d, and &P < 0.05 vs. 14d.
