Figure 4
From: In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing
Impact of SOCS-4 knockdown on cellular migration and tubule formation. Cellular migration was assessed using an ECIS model in (a) HaCaT and (b) HECV cells following SOCS-4 knockdown. Mean change in resistance following monolayer wounding was detected by the ECIS system and was used to quantify cell migration. Cellular migration was also explored using a conventional scratch wound assay, monitored by the EVOS system, in (c) HaCaT and (d) HECV cells following SOCS-4 knockdown. Angiogenic potential was assessed, following SOCS-4 knockdown in HECV cells using a Matrigel tubule formation assay (e). Data shown is representative of a minimum of three repeats, error bars represent standard deviation (a/b/e) and standard error of the mean (c/d), * represents p < 0.05, ** represents p < 0.01, *** represents p < 0.001 and **** represents p < 0.0001.
