Figure 6
From: In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing
Impact of SOCS-4 suppression on the proliferative and migratory responses of HaCaT keratinocytes to EGF and TGFβ. Proliferative rates of (a) HaCaT pEF6 and (b) HaCaT SOCS-4 knockdown cells in addition to 20 ng/ml EGF or 2 ng/ml TGFβ were assessed using a MTT growth assay over 3 and 5 days. Cellular migration in response to 20 ng/ml EGF and 2 ng/ml TGFβ was also explored using a conventional scratch wound assay on (c) HaCaT pEF6 and (d) HaCaT SOCS-4 knockdown cells. Data shown are mean values of a minimum of three independent repeats, error bars represent the standard error of the mean and * represents p < 0.05.
