Table 1 Primers/sequences used in the study.
From: In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing
Target gene/Sequence | Forward | Reverse |
|---|---|---|
SOCS-1 | 5′-GATGGTAGCACACAACCAG | 5′-ACTGAACCTGACCGTACAGAGGAAGAGGAGGAAGGTT |
SOCS-2 | 5′-GTCAGACAGGATGGTACTGG | 5′-CTGGAATTTATATTCTTCCAA |
SOCS-3 | 5′-AAGACCTTCAGCTCCAAGA | 5′-GTCTTCCGACAGAGATGCT |
SOCS-4 | 5′-CAGCTGTTCATCCATTGAG | 5′-CCATTTGGGTTTGTTTCTT |
SOCS-5 | 5′-AGTCAAAGCCTCTCTTTTCC | 5′-ACTGAACCTGACCGTACACATTTTGGGCTAAATCTGA |
SOCS-6 | 5′-CCTTACAGAGGAGCTGAAAA | 5′-ACTGAACCTGACCGTACACGAAAAGAAAAGAACCATC |
SOCS-7 | 5′-AGACTAACAGCTGCTCGGAA | 5′-CCCACTGATATCATCTAGGAGGC |
GAPDH | 5′-GGCTGCTTTTAACTCTGGTA | 5′-GACTGTGGTCATGAGTCCTT |
SOCS-3 (q-PCR) | 5′-TCAAGACCTTCAGCTCCA | 5′-ACTGAACCTGACCGTACAGTCACTGCGCTCCAGTAG |
SOCS4 (q-PCR) | 5′-GGCAGTGTTTTCCAATAAAG | 5′-ACTGAACCTGACCGTACAAGGTGGGAAAGGACACTTAT |
GAPDH (q-PCR) | 5′-CTGAGTACGTCGTGGAGTC | 5′-ACTGAACCTGACCGTACACAGAGATGATGACCCTTTTG |
SOCS-4 (ribozyme transgene) | 5′-CTGCAGTTCACTGATATGAATTTTCCTTTTAGACGCTGATGAGTCCGTGAGGA | 5′-ACTAGTCGGCACTCTTCAGGGCTTTTCGTCCTCACGGACT |
SOCS-3 (coding sequence amplification) | 5′-ATGGTCACCCACAGCAAGTT | 5′-TTAAAGCGGGGCATC |
