Figure 4 | Scientific Reports

Figure 4

From: Tumor-Derived Exosomes Induce the Formation of Neutrophil Extracellular Traps: Implications For The Establishment of Cancer-Associated Thrombosis

Figure 4

Tumor-derived exosomes induce NET formation. (A) Histogram indicating the size distribution of exosomes isolated from the culture supernatants of 4T1 cells. Exosomes were isolated and quantified as described in the Methods section. Bone marrow cells isolated from G-CSF-treated mice were incubated for 3 h at 37 °C in the absence (B) or presence (C) of 0.1 µg of 4T1-derived exosomes (Exo). Immunofluorescence staining for DNA (blue), Ly6G (green) and citrullinated histone (red) was performed as described in the Methods section. Scale bars = 20 μm. (D) The quantification of NETs was performed as described in the Methods section. **P < 0.01; unpaired, two-tailed Student’s t-test. Experiments were run in triplicate (n = 3).

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