Figure 6 | Scientific Reports

Figure 6

From: Tumor-Derived Exosomes Induce the Formation of Neutrophil Extracellular Traps: Implications For The Establishment of Cancer-Associated Thrombosis

Figure 6

G-CSF and tumor-derived exosomes accelerate venous thrombosis in tumor-free mice. (A) Neutrophil counts in the peripheral blood of control (n = 5) and G-CSF-treated (n = 5) mice. (B) The venous thrombosis model was applied to control mice (●, n = 12), mice treated with G-CSF (■, n = 6), control mice infused with 100 µg of 4T1-derived exosomes (▲, n = 3), or mice treated with G-CSF infused with 100 µg of 4T1-derived exosomes (▼, n = 5). The data represent the mean occlusion time after photochemical-induced vascular injury, with each data point representing one individual mouse. **P < 0.01; ***P < 0.001; analysis of variance (ANOVA) with Tukey’s posttest. (C) Fluorescence microscopy analysis of cryosections of venous thrombi from control mice treated with exosomes (upper) and mice treated with G-CSF + exosomes (bottom). (D) Higher magnification of the venous thrombi from control mice treated with exosomes (upper) and mice treated with G-CSF + exosomes (bottom). The sections were stained with Ly6G (green) and Hoechst (blue). Extracellular DNA fibers are indicated by arrows. Bars = 50 μm.

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