Figure 4

Representative inter-sample comparisons at the single-miRNA level. (A) Uninfected horse #1 vs. uninfected horse #2. (B) Infected horse #1 vs. infected horse #2. (C) Uninfected horse #1 vs. infected horse #1. Points represent DESeq2-normalized read counts for each miRNA and are drawn in log₁₀ scale on both axes. The midline (\( \mbox{-} \mbox{-} \mbox{-} \)) illustrates optimal correlation (i.e. points that fall on this line have equal expression in both samples). In contrast, points that lie further from the midline represent between-sample variation for individual miRNAs. R ² values reflect the overall correlation between samples, while the line of best fit (red line) was determined using linear least-squares regression.