Figure 2
From: TDP-43 stabilises the processing intermediates of mitochondrial transcripts
TDP-43 is localised in mitochondria in T-REx 293 cells. (a) Mitochondria prepared from T-REx 293 cells (T-REx 293 MT) or DAP-TDP-43 expressing T-REx 293 cells (TDP-43 MT) were treated with proteinase K (PK) in the presence (+) or absence (−) of Triton X-100 (TX-100). Proteins present in the outer mitochondrial membrane (OMM), inner membrane (IMM), or matrix were detected by WB with antibodies indicated on the right. Open arrowhead and closed arrowhead indicate DAP-TDP-43 and endogenous TDP-43, respectively. TCL; Total cell lysate. (b) Endogenous TDP-43 was detected within mitochondria of T-REx 293 cells by immunoelectron microscopy using anti-TDP-43 (60019-2-Ig). Open arrowheads indicate stained TDP-43. N, nucleus; mt, mitochondria.
