Figure 2
Intratracheal administration of IL-33 induced infiltration of eosinophils in the lung. IL-33 or saline (control) was administered intratracheally to BALB/c mice on Day 0, and the indicated assays were performed on Days 3 to 14. (A) The BAL fluid was assessed at the indicated time point. The numbers of total leukocytes (left), eosinophils (middle), and lymphocytes (right) in the BAL fluid are shown. (B) Microscopic pictures of the lungs from mice as in A, fixed and stained with Giemsa reagent. Scale bars: 50 μm (top and 3rd line), 10 μm (2nd and bottom line) and 5 μm (right panel of 2nd and bottom line). (C) Histological scores from Giemsa-stained samples are depicted. (D) The vascular permeability was determined by the concentration of albumin in BAL fluid. Representative data were obtained from four independent experiments. Mean values ± SD are shown for (A) and (D). *p < 0.05: one-way ANOVA.
