Figure 6 | Scientific Reports

Figure 6

From: The neuroprotective effect of hesperidin in NMDA-induced retinal injury acts by suppressing oxidative stress and excessive calpain activation

Figure 6

Hesperidin ameliorated cleavage of α-fodrin in the retina after NMDA injury. (a) Immunoblot analysis of α-fodrin in retinas with or without hesperidin treatment, 1, 3, 6 and 12 hours after NMDA injury. Representative immunoreaction image with anti-α-fodrin showing intact α-fodrin (280 kDa), caspase-3-cleaved fragmented α-fodrin (150 kDa) and calpain-cleaved fragmented α-fodrin (145 and 150 kDa). β-actin was used as an internal control. (b) The relative density of the cleaved-fodrin immunoreactive band. Relative density was based on the cleaved-fodrin immunoreactive band one hour after NMDA injection. Full-length blots are shown in Supplementary Figure 6. Data represent mean ± SD (no treatment and 3 h after NMDA: n = 5, other groups: n = 6 each). *p < 0.05, **p < 0.01, ***p < 0.001.

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