Figure 3
From: Bone matrix components activate the NLRP3 inflammasome and promote osteoclast differentiation
Bone matrix components stimulate OC differentiation. BMM from WT or Nlrp3 −/− mice were incubated with 25 ng/ml RANKL for 2 days (D2) then treated with bone particles. (a) Cells were stained for TRAP on day 3 (D3). Original magnification, ×2. (b) OC number/well. (c) IL-1β production was measured in the supernatants from (b). (d) Effects of IL-1 receptor deficiency on OC formation. Experiments were run in triplicates, and data are means ± SD. (b) **p = 0.0013, ****p < 0.0001; (c) WT: ****p < 0.0001; Nlrp3 −/−: **p = 0.0030, ****p < 0.0001; (d) ***p = 0.0008; ****p < 0.0001. p values correspond to significant differences relative to the control for each genotype, and data are representative of at least 4 independent experiments.
