Figure 2

STx of ZIKV in AIR mice. (A) In two replicate experiments, AIR male and female mice were infected with ZIKV intraperitoneally (i.p.). At 8 dpi, these male and female AIR mice were mated/co-housed with Ifnar1 ^−/− female mice for 4 days at the times indicated. (B,C) Ifnar1 ^−/− female mice were then monitored for pregnancy and weight loss for 20 days post mating (dpm). Data are shown as individual percent starting body weight of Ifnar1 ^−/− females bred with AIR mice. Plots are color-coded to indicate weight loss groups. Ψ indicates mice euthanized due to clinical disease. Two mice became pregnant during these experiments and are shown in Supplementary Figure 3. (D–F) At 20 dpi, (D) brain, (E) spleen and (F) plasma from Ifnar1 ^−/− mice were analyzed for viral RNA or NAb. (D–E) quantitative real-time (qRT) PCR analysis of individual mice for viral RNA in (D) brain and (E) spleen. Dotted line indicates sensitivity level of assay for each tissue. (F) Inhibitory dilution of neutralizing antibody (NAb) for each animal. No NAb titer was detected for the mouse that was mated with no weight loss (green symbol) or co-housing control mice. One mouse could not be tested in the mated (clinical) group due to lack of plasma sample.