Figure 3
Detection of virus in vaginal tissues following STx. (A–B) Weights of (A) clinical and (B) non-clinical mice described in Table 1. Data are shown as individual percent starting body weight, similar to that shown in Fig. 2B–C. (A) Mouse in red symbol/line (XZ188-2) was determined to be pregnant at the time of clinical disease. (C–E) ISH and (F) IHC of (C,E) uterus and (C,D,F) placental tissue from XZ188-2. (C) Low magnification of uterus, placenta and fetus from XZ188-2 stained for genomic RNA (brown). Red line is drawn to indicate fetal tissue, which was not positive for either genomic or intermediate viral RNA. (D–E) Higher magnification of (D,Di) placental and fetal or (E,Ei) uterus tissue showing detection of ZIKV (D,E) genomic RNA or (Di,Ei) intermediate RNA. Intermediate sections were stained in adjacent sections to those used for genomic RNA. (F) Placenta tissue from XZ188-2 showing viral protein (NS5) as well as active Caspase 3 staining (Casp 3) in localized areas of the placenta.
