Figure 5
ZIKV infection in pups/fetuses from infected pregnant AIR mice. (A) Low magnification image of ZIKV ISH labeled section with nuclear counterstain of a whole-mount fetus (~E18) and associated placenta from an infected, pregnant AIR mouse. Notice the intense labeling in the placenta on the left side of the image. Areas outlined in boxes correspond to high magnification images of ZIKV positive cells in the (B,C) pretectum of the brain and the (D) pharyngeal/cervical region of the embryo. Dark signal associated with the eye is the developing retinal pigment epithelium and not ZIKV RNA as control animals also contained this signal (not shown). The scale bar shown in (A) pertains to (A–D). (E,F). IHC of medulla tissue from a neonatal pup. Tissue was labeled with antibodies against ZIKV NS5 (green) and co-stained with (E) Sox2 (magenta) or (F) NeuN (magenta). DAPI stain was used to visualize cellular nuclei. Scale bars are labeled.
