Table 1 Detection of virus in vaginal swabs of Ifnar1 ^−/− mice mated to ZIKV-infected AIR males.

Mouse #^a	0^b	5	9–10 ^c	12–14 ^d	Clinical^e	NAb^g
XZ185-1	−	−	−	−	−	0
XZ185-2	−	+	−	na	9	1:6,250
XZ186-1	−	−	++	++	12	1:6,250
XZ186-2	−	−	++	−	−	1:1,250
XZ187-2	−	++	+	na	9	1:6,250
XZ188-1	−	−	−	−	−	0
XZ188-2	−	+	+	na	10	1:6,250
XZ189-1	−	−	+	−	12	1:6,250
XZ189-2	−	−	−	−	−	0
XZ190-1	−	++	++	na	10	1:6,250
XZ190-2	−	−	−	−	−	0
XZ190-3	−	−	−	−	−	0

^aItalicized mouse (XZ188-2) was impregnated by an infected AIR male (Fig. 3).
^bVaginal swabs from mice at indicated dpm were analyzed for virus by RNA as described in methods. – indicates that CT values for viral RNA were comparable to mock controls (no detection below 29 cycles).; +indicates low detection of virus with CT values between 26–29; ++ indicates significant viral RNA with CT values less than 26.
^cAll mice were analyzed at 10 dpm except XZ185-2 and XZ187-2 which were analyzed at 9 dpm.
^dMice were analyzed at 14 dpm except XZ186-1, which was analyzed at 12 dpm, and the four mice that had already developed clinical disease and were no longer in the study (NA).
^edpi when clinical disease was observed; (−) not clinical.
^fViremia was tested for in all mice, but was not detected.
^gNAb titers in plasma from indicated mice. Serial 5 fold-dilutions were completed. Highest dilution that inhibited 50% of virus infection is reported.

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