Figure 6
From: Characterisation of lung macrophage subpopulations in COPD patients and controls
Expression of CXCL1 and TLR3 protein and phagocytosis of pHrodo E. coli BioParticles in macrophage subpopulations. Alveolar (AM) and interstitial (IM) macrophages were isolated using EasySep monocyte enrichment kit. Cells were used to generate cytospin slides (A and B) or exposed to pHrodo E. coli BioParticles for 1 h in a shaking incubator (C and D). Percentage of small alveolar, large alveolar and small interstitial macrophages expressing CXCL1 protein and TLR3 protein are shown in (A and B) respectively. Data represents mean (SEM) of n = 6 COPD patients. Phagocytosis was analysed by flow cytometry and is represented by (C): % of pHrodo bright macrophages within each subpopulation and (D): median fluorescence intensity (MFI) of each subpopulation relative to the negative control. Data are shown as mean (SEM) of n = 9 COPD patients One way ANOVA followed by Tukey-Kramer Multiple Comparisons Test was performed. *,**,***Significantly different compared to expression of the same protein on large alveolar macrophages (p < 0.05, < 0.01 and <0.001 respectively). #,##Significantly different compared to the percentage of pHrodo bright macrophages or MFI of other macrophage subpopulations (p < 0.05, <0.01 respectively).
