Figure 6

Automated western blotting of selected CSF proteins. Five proteins (cathelicidin (panel c), ceruloplasmin (panel d), myeloperoxidase (panel e), protein S100A9 (panel f) and cystatin C (panel g) were quantified in a larger cohort (n = 40, 15 hospital controls, 5 healthy controls and 20 SPP patients) using automated capillary western blotting (Wes, ProteinSimple, CA, USA). A typical ‘pseudo-gel image is shown in panel a, highlighting the position of the GST:cathelicidin fusion standard protein and the native cathelicidin the corresponding standard curve (panel b). Purified recombinant proteins were used as calibration standards for all five proteins. Panels c to g are the summarised quantitative western blot data for cathelicidin, ceruloplasmin, myeloperoxidase, S110A9 and cystatin C respectively. Dots show individual results for SPP samples (red) and hospital controls (blue) and healthy controls (purple). Top and bottom of the box represent the 75% (Q3) and 25% (Q1) percentile, the band inside the box is the median and whiskers extend to 1.5 times the interquartile range from the box. Points outside the box are outliers. For each protein, t-tests were used to assess the differences between samples.