Table 3 Effect of TUDCA supplementation during embryo culture on the quality of blastocysts.

From: Tauroursodeoxycholic acid enhances the development of porcine embryos derived from in vitro-matured oocytes and evaporatively dried spermatozoa

Groups

No. of blastocyst

ICM (mean ± SD)

TE (mean ± SD)

Total (mean ± SD)

ICM/Total (%, mean ± SD)

FT

30

5.1 ± 0.1a

25.9 ± 0.4a

31.0 ± 0.4a

16.6 ± 0.4a

ED

30

3.6 ± 0.1b

21.4 ± 0.4b

25.0 ± 0.4b

14.4 ± 0.5b

ED+ TUDCA

30

7.0 ± 0.2c

27.5 ± 0.1c

34.5 ± 0.3c

20.3 ± 0.3c

  1. Note: Different letters within the same column indicate statistical differences at p < 0.05 by one-way ANOVA followed by the LSD multiple comparison test (N = 3). FT: blastocysts developed from conventional freeze-thaw boar spermatozoa without TUDCA-treatment; ED and ED+ TUDCA: blastocysts developed from evaporatively dried boar spermatozoa with 0 and 200 μM TUDCA-treatment, respectively. ICM: cell numbers of the inner cell mass; TE: numbers of trophoblast cells; Total: cell numbers of the inner cell mass plus numbers of trophoblast cells.
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