Table 4 Primers used for RT-PCR analyses.

From: Tauroursodeoxycholic acid enhances the development of porcine embryos derived from in vitro-matured oocytes and evaporatively dried spermatozoa

Gene

Primers (5′-3′)

Product size (base pair)

RT-PCR condition

BAX

F: AAGCGCATTGGAGATGAACT R: GGCCTTGAGCACCAGTTTAC

184

40 cycles/57 °C

BAK

F: CTGCCCCTAGAACCTAGCAG R: TTGATGCCACTCTCGAACAG

186

40 cycles/57 °C

BCL-2

F: ATGTGTGTGGAGAGCGTCAA R: CCTTCAGAGACAGCCAGGAG

188

45 cycles/57 °C

BCL-XL

F: ACAGCGTATCAGAGCTTTGAGCA R: CGTCAGGAACCATCGGTTGAAG

297

35 cycles/58 °C

GAPDH

F: TTCCACGGCACAGTCAAGGC R: CATGGTCGTGAAGACACCAG

151

40 cycles/57 °C

  1. Note: F: forward primer; R: reverse primer.
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