Figure 5

The mlo2 mlo6 mlo12 triple mutants show enhanced disease symptoms upon challenge with F. oxysporum. Two-week-old Arabidopsis seedlings were inoculated with a spore suspension of F. oxysporum (isolate Fo5176). (A) Infection phenotypes were scored at 5, 7 and 10 dpi by assigning a disease index on a 0 (no symptoms) to 5 (severe disease symptoms) scale. Data shown are from a representative experiment and based on 15-20 seedlings per genotype. Each symbol in the categorical scatter plot (circle, square or triangle) represents the infection phenotype of one seedling. The crosses indicate the mean values ± SEM. *, ** and *** indicate statistically significant differences from Col-0 (P < 0.05, P < 0.01 and P < 0.001, respectively) according to a GLM test (Poisson distribution) on n = 15-20 technical replicates (individual seedlings). The experiment was repeated twice with similar results (Fig. S2). (B) Representative macroscopic infection phenotypes at 7 dpi.