Figure 6

The mlo2 mlo6 mlo12 triple mutants show an elevated bacterial titre upon challenge with P. syringae. Five-week-old Arabidopsis plants were infiltrated with P. syringae pv. maculicola lux (OD₆₀₀ = 0.0005) and luminescence (RLU cm⁻²; corresponding to bacterial titre) determined at 0 dpi (to ensure an equal start inoculum) and 3 dpi. (A) The boxplot shows data from one representative experiment based on n = 7 (0 dpi) and n = 11 plants (3 dpi) per genotype, with each plant value representing the mean of three leaves. Centre lines mark the medians, upper and lower box limits indicate the 25^th and 75^th percentiles, respectively; upper and lower whiskers extend 1.5 times the interquartile range from the 25^th and 75^th percentiles, respectively; and dots represent outliners. Letters indicate statistically different groups (at least P < 0.05) according to a GLM test (quasi-Poisson distribution) on n = 11 technical replicates (individual plants). The experiment was repeated four times with n = 7-13 plants per genotype in each experiment and in two different controlled environments (see Materials and Methods for details) with comparable results (Fig. S3). (B) Representative macroscopic infection phenotypes at 3 dpi. Yellow arrows indicate the inoculated leaves.