Figure 3 | Scientific Reports

Figure 3

From: Methylation profile of a satellite DNA constituting the intercalary G+C-rich heterochromatin of the cut trough shell Spisula subtruncata (Bivalvia, Mactridae)

Figure 3

PCR amplification and Southern blot hybridisation of SSUsat in different bivalve species. Agarose gel electrophoresis of the SSUsat fragments obtained after PCR amplifying genomic DNA of Spisula subtruncata (SSU), Spisula solida (SSO), Mactra stultorum (MST) and Donax trunculus (DTR) using primers derived from the SSUsat consensus sequence (a). Corresponding Southern blot hybridisation using an SSUsat probe (b). Blank represents PCR reaction without DNA template. (c) Scheme of tandemly organized SSUsat monomers showing the locations of the inversely orientated PCR primers (SSUsatPR1 in red, SSUsatPR2 in green).

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