Figure 4 | Scientific Reports

Figure 4

From: Intravenous infusion of human bone marrow mesenchymal stromal cells promotes functional recovery and neuroplasticity after ischemic stroke in mice

Figure 4

hBM-MSC promote neuronal survival in the perilesional area. (A) Stroke induces a similar atrophy in tMCAo hBM-MSC and tMCAo PBS mice 5w post-injury. (B) Representative micrograph shows typical brain atrophy and displays the ROI selected for histopathological analyses. Quantification of neuronal death in perilesional cortex (red box) indicates a neuronal rescue after hBM-MSC administration in stroke mice compared to tMCAo PBS mice (C,D). No difference is observed when a medial ROI (blue box) is selected (E,F). (G) Immunofluorescence analysis in perilesional cortex shows a higher percentage of PV+ neurons in tMCAo hBM-MSC compared to tMCAo PBS mice. (I) The percentage of PV+ cells in medial cortex is comparable in tMCAo hBM-MSC and tMCAo PBS mice. (H,J) Microphotographs show PV stained cells in red box (Ha,b) and in blue box (Ja,b). Data are mean + SD, n = 6–10, unpaired t-test. *p < 0.05. Bar = 50 µm.

Back to article page