Table 2 Combinations of fluorochrome-conjugated monoclonal antibodies used for the immununophenotypic characterization of hBM-MSC.
Tube n | Fluorochrome | |||
|---|---|---|---|---|
FITC | PE | PerCP | APC | |
1 | unstained | unstained | unstained | unstained |
2 | CD34 (IQP) | CD90 (eBiosciences) | CD45 (Becton Dickinson) | CD274 (PDL1) (Biolegend) |
3 | HLA-DR (IQP) | CD105 (eBiosciences) | CD45 (Becton Dickinson) | CD106 (VCAM) (Becton Dickinson) |
4 | HLA-ABC (Becton Dickinson) | CD33 (Becton Dickinson) | CD45 (Becton Dickinson) | CD44 (eBiosciences) |
5 | CD14 (IQP) | CD73 (BD Pharmingen) | CD45 (Becton Dickinson) | CD19 (Becton Dickinson) |
6 | CD146 (eBiosciences) | PDGFRβ (Becton Dickinson) | CD45 (Becton Dickinson) | NG2 (R&D) |
7 | CD4 (Becton Dickinson) | CD54 (Becton Dickinson) | CD45 (Becton Dickinson) | CD11b (MAC-1) (Becton Dickinson) |
