Figure 3 | Scientific Reports

Figure 3

From: A conserved neuronal DAF-16/FoxO plays an important role in conveying pheromone signals to elicit repulsion behavior in Caenorhabditis elegans

Figure 3

Expression pattern of glna-3P:gfp and ChIP analysis of DAF-16/FoxO::GFP bound to the upstream region of glna-3 gene (a) and (b) Expression pattern of glna-3P::gfp. Worms were also stained with DiI dye to visualize chemosensory amphid neurons. (c) str-1P::glna-3 rescued daf-16/FoxO mutant phenotype (wild type, n=160; daf-16/FoxO, n=155; str-1P::glna-3; daf-16/FoxO, n=150.) *P<0.05 compared to wild type, **P<0.05 compared to daf-16/FoxO. (d) Putative DAF-16/FoxO binding sites in the 5′ upstream region and first intron of the glna-3 gene. (e) ChIP of DAF-16/FoxO::GFP with anti-GFP antibody in daf-2;DAF-16/FoxO and daf-2;DAF-16/FoxO;DAF-16/FoxO::gfp mutants. Bars represent the mean of three independent biological replicates. *P<0.05 compared to daf-2;DAF-16/FoxO. Significance was determined using two-tailed, unpaired t-tests.

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