Figure 1
From: The anti-influenza M2e antibody response is promoted by XCR1 targeting in pig skin
Selective targeting of pig skin cDC1 subset with poXCL1-mCherry upon intradermal injection with different adjuvant formulations. NIP-mCherry and poXCL1-mCherry (60 µg) were injected intradermally in different adjuvant formulations, skin cells were collected after 24 h by enzymatic dissociation and labeled for MHC class II and CD172A detection. FSChigh and MHC class IIhigh cells (cDC) were gated as shown in A (left panel) and their percentage among total extracted skin cells is indicated. The FSChigh and MHC class IIhigh gated cells were analyzed for CD172A and mCherry expression (in A (right panel) to E) and the percentage of each quadrant among gated cells is indicated. The injections are: in A (right panel), PBS; in B, plain VB; in C, VB formulated with SWE adjuvant; in D, VB formulated with CL + MPL; in E, VB formulated with NL + MPL.
