Figure 2
From: The anti-influenza M2e antibody response is promoted by XCR1 targeting in pig skin
Characterization of poXCL1-M2e and NIP-M2e VB. In (A) the M2e VB consist of a targeting unit (poXCL1 or NIP), a dimerization domain with hinge and CH3 from human IgG3 and an antigenic unit with three repeats of the M2e sequence. In (B) purified poXCL1-M2e (predicted size 33 kDa) and NIP-M2e (predicted size 48 kDa) were analyzed by SDS-PAGE under reducing (+DTT) and non-reducing (-DTT) conditions. In (C) normalized concentrations of purified poXCL1-M2e and NIP-M2e preparations were evaluated by ELISA, and detected using an antibody specific for the VB dimerization domain.
