Figure 3 | Scientific Reports

Figure 3

From: Phaeocystis globosa Virus DNA Polymerase X: a “Swiss Army knife”, Multifunctional DNA polymerase-lyase-ligase for Base Excision Repair

Figure 3

DNA substrate preference for PgVPolX. The following different molecules used in the analysis: T/P, template-primer; Gap-5′/OH, 5 nucleotides gap; and Gap-5′/P, 5 nucleotides gap bearing a 5′-phosphate group are schematized on top of the figure. The assay was carried out as described in Materials and Methods with 25 nM DNA substrate and 100 µM dNTPs. Asterisks indicate the 5′ Cy5-labeled end of the primer strand.

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