Figure 3 | Scientific Reports

Figure 3

From: TGFβ signaling is associated with changes in inflammatory gene expression and perineuronal net degradation around inhibitory neurons following various neurological insults

Figure 3

Perineuronal nets around PV(+) interneurons are degraded following traumatic brain injury. (a) Time line of experimental design. (b) Representative confocal images of rat undercut and contralateral (Cont) cortices stained for parvalbumin (PV) and perineuronal nets using Wisteria Floribunda agglutinin (WFA) 7 days after the operation. Scale bar = 50 µm. White arrowheads indicate representative PV(+)/WFA(+) cells. Areas within white rectangles in merged images are shown magnified. (c) The percentage of PV(+) interneurons associated with PNNs was significantly decreased at 7 days following the undercut operation. *p < 0.05, Two-way ANOVA with post-hoc Sidak’s test, two tailed. Three biological replicates (n’s = 3) were used at each time point.

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