Figure 4 | Scientific Reports

Figure 4

From: TGFβ signaling is associated with changes in inflammatory gene expression and perineuronal net degradation around inhibitory neurons following various neurological insults

Figure 4

Albumin activated the transcriptional responses of ECM genes via TGFβ receptor signaling. (a) The expression levels of ECM-related genes is upregulated across models (marked in bold in A, C) in a separate microarray data set in comparisons between albumin vs. albumin + TGFβ receptor (R) blockers. **p = 0.0078, Wilcoxon signed-rank test, two-tailed. (b–g) Quantitative real-time PCR for the mRNA expression of selected genes in primary cortical astrocytic or neuronal cultures at 24 hours following treatment of albumin or albumin plus a specific Alk5 blocker, SJN2511. Results were of three independent primary culture derivations (n = 11 per condition in neurons; n = 10–12 per condition in astrocytes). One way ANOVA with posthoc Turkey’s test (in astrocytes) and student t-test (in neurons) were performed. Cont, control; Alb, albumin; SJN, SJN2511; n.d., non detectable (Cq value ≥ 35). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data are shown as mean ± S.E.

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