Figure 2 | Scientific Reports

Figure 2

From: A Low Permeability Microfluidic Blood-Brain Barrier Platform with Direct Contact between Perfusable Vascular Network and Astrocytes

Figure 2

Comparison of three neuron cultures in NBMB27, NBMFBS, and EGM for assessing the effect of FBS in neural cultures. (a) Immunostained images of each experimental medium culture. (b) The total number of nuclei and synapses identified in each culture. As the neurons were grown in a mass against the fibrin hydrogel wall and do not migrate, synaptic connections were measured at the cell mass. (c) The per neuron average of synaptic connections value for NBMFBS was found to be similar to that of cultures grown in NBMB27 control. EGM supplied neuronal cultures show a drastic decrease in synaptic connectivity. All data show mean ± SEM. Statistical analysis student’s t-test. **p < 0.01. 12 ROI for each condition were used for analysis.

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