Table 1 Information on the heterologous genes and their chromosomal insertion sites in P. putida KT2440.

From: Combinatorial metabolic engineering of Pseudomonas putida KT2440 for efficient mineralization of 1,2,3-trichloropropane

Gene

Length (bp)

Amino acid residues

Function

Strains (GenBank accession no.)

Insertion site/targeted deletion

dhaA90R

882

294

haloalkane dehalogenase

Rhodococcus rhodochrous NCIMB13064 (AF060871)

PP_1277 (algA)

hheC

765

255

haloalcohol dehalogenase

Agrobacterium radiobacter AD1 (AF397296)

PP_1278/PP_1279 (algF/algJ)

echA

885

295

epoxide hydrolase

Agrobacterium radiobacter AD1 (Y12804)

PP_1280 (algI)

glpR

756

252

glycerol-3-phosphate regulon repressor

Pseudomonas putida KT2440 (AE015451)

PP_1074

vgb

441

147

hemoglobin

Vitreoscilla sp. HG1 (AF292694)

PP_3356 (fcs)

flagellar operon

68139

69 genes

flagella synthesis

Pseudomonas putida KT2440 (AE015451)

PP_4329-PP_4397