Figure 1
From: TIA1 is a gender-specific disease modifier of a mild mouse model of spinal muscular atrophy
Gender-specific modulation of body weight with Tia1 knockout. (A) Diagrammatic representation of the first 104 nucleotides of SMN2 intron 7 (numbering begins from intron 7 beginning). Positive cis-elements (TIA1 and Element 2) that promote exon 7 inclusion and negative cis-element that promote exon 7 skipping (ISS-N1) are indicated by (+) and (−), respectively. hnRNPA1/A2 binding sites are also shown. Diagram adapted from20. (B) Representative semi-quantitative radioactive RT-PCR gel showing the results of MESDA35 to determine the abundance of SMN2 splice variants. Tissue and mouse genotype are indicated above, lane numbers are indicated below and isoforms are indicated to the right of the gel, respectively. The graph presents the results of densitometry (n = 2 mice per tissue, sex and genotype). The intensity for each isoform band is expressed as a percent of the total intensity for each lane. Since there were no significant differences between values for male and female mice, values from both sexes were combined when calculating relative abundances of isoforms. (C) BW from P1 until P59 for male (left) and female (right) mice. For male mice, n = 5, 12, 6 and 7 mice for WT, Tia1 −/−, C +/+ and C +/+/Tia1 −/− genotypes, respectively. For female mice, n = 5, 4, 6 and 5 mice for WT, Tia1 −/−, C +/+ and C +/+/Tia1 −/− genotypes, respectively. Statistical comparison between the genotypes was performed with a repeated-measures two-way ANOVA followed by Tukey’s multiple comparison test. Details about these statistical comparisons appear below the graphs. For each sex, the p value indicates the result of Tukey’s multiple comparison test and the Ages column indicates at which ages the weight was significantly different between the specified genotypes. (D) Percent weight gain for male (left) and female (right) mice from P1 until P59 determined by dividing the P59 by the P1 weight. Errors bars on all graphs indicate S.E.M. For (D), statistical comparison between genotypes of the same sex was performed using a one-way ANOVA with Tukey’s multiple comparisons test and the results are denoted by lowercase letters. Bars with different letters indicate a statistically significant different between the means, whereas bars with the same letters are not significantly different (p < 0.05).
