Figure 1
4bHWE modulates the alternative splicing of apoptotic gene transcripts. Cells were treated with 4bHWE at the indicated concentration for 24 h. (a) Chemical structure of 4bHWE. (b) Cell viability of Huh-7 cells and human fibroblasts were analyzed through MTT assays. (c) mRNA was extracted and detected using RT-PCR for the alternative splicing of the HIPK3, SMAC/DIABLO, and SURVIVIN transcripts in Huh-7 (second row), A549 (third row), and GBM8401 (fourth row) cells. The splicing isoforms are illustrated in the top row, and their expected PCR products derived through the primers are indicated by arrowheads. The ratios of the densities of the two bands (alternative exon-containing isoforms to alternative exon-lacking isoforms) are presented below each group. Data are the mean of three independent experiments. *p < 0.05 compared with the control. M = marker.
