Figure 5
4bHWE affects genome-wide alternative splicing detected using RNA sequencing. Huh-7 cells were treated with (a) 0.1% DMSO and (b) 10 μM 4bHWE for 24 h prior to RNA sequencing. Grey bars indicate the number of alternative splicing events and white bars indicate the number of genes in which alternative splicing events occurred. The number shown above the bar is the event. This experiment was performed once. (c) Huh-7 cells were treated with the indicated concentration of 4bHWE for 24 h and then harvested. mRNAs were extracted and detected using RT-PCR for the alternative splicing of APAF1, CCAR1, and RIPK1 transcripts. The splicing isoforms are illustrated in the left column, and their expected PCR products using the primers are indicated by arrowheads. The ratios of the densities of the two bands (alternative exon-containing isoforms to alternative exon-lacking isoforms) are presented below each group. Data are the mean of three independent experiments. *p < 0.05 compared with the control. M = marker.
