Figure 2
BML-111 attenuates the translocation and expression of HMGB1 in IMQ-induced psoriasiform dermatitis in mice. (A) Immunofluorescence staining of HMGB1 (green) in skin lesions of mice. Nuclei were counterstained with DAPI (blue). (B) Cytoplasmic extract (CE) and nuclear extract (NE) from the epidermal cells in the lesional skin of mice were used for western blots analysis of the indicated proteins. Full-length blots are presented in Suppl. Figure S1. (C) Ratio of HMGB1 expression in CE to NE was assessed by the quantification of relative optical density. (D) HMGB1 mRNA levels in separated epidermis of mouse skin biopsies were evaluated using PCR. The results are shown as the mean ± SD. **p < 0.01 and *p < 0.05, compared to mice treated with IMQ. All experiments were conducted thrice, and the representative results are shown.
