Figure 4

MUC1-C expression in MCF7 and MDA-MB231 cells. MUC1-C expression in MCF7 (a–e) and MDA-MB231 cells (f–j) without drugs or (a,f) with cyclophosphamide (b,g), doxorubicin (c,h), epirubicin (d,i) or 5-fluorouracil (e,j). Note the higher expression of MUC1-C in the MCF7 cells than that in the MDA-MB231 cells, but no changes were observed with the chemotherapy drug treatments. The MUC1-C antibody was diluted 1/400 (40x magnification).