Figure 5

Serum analysis and histomorphometric evaluation of posttraumatic bone turnover in ovariectomized mice fed a control (C), Ca/VitD-deficient (D) or Ca/VitD-supplemented (S) diet. (a) Serum parathyroid hormone (PTH) concentrations of fractured mice on day 10 and 23 post-fracture (n = 6–8/group). (b) Number of osteoclasts per bone perimeter (N.Oc/B.Pm), (c) osteoclast surface per bone surface (Oc.S/BS), (d) number of osteoblasts per bone perimeter (N.Ob/B.Pm), (e) osteoblast surface per bone surface (Ob.S/BS), (f) bone formation rate per bone surface (BFR/BS), (g) mineral apposition rate (MAR) and (h) mineralized surface per bone surface (MS/BS) of fractured mice relative to non-fractured mice of the same dietary group (dashed line) in percent examined in lumbar vertebrae (L2) on day 23 (n = 7–9/group). (i) Representative images of lumbar vertebrae stained with tartrate-resistant acid phosphatase (TRAP) of fractured and non-fractured mice. Data presented as the mean ± SD. Significant differences between C, D and S: *p < 0.05, **p < 0.01, ***p < 0.001 evaluated by ANOVA/Fishers LDS post-hoc. ^#Significantly different (p < 0.05) from non-fractured mice of the same dietary group (dashed line) evaluated by Student’s t-test.