Figure 4
From: Innate Immune Basis for Rift Valley Fever Susceptibility in Mouse Models
Changes in the percentages of innate immune cells in RVFV-infected BALB/c and MBT mice. (a) A comprehensive flow cytometric profiling of leukocytes defined as positive for CD45 in the blood, spleen, liver and bone marrow of RVFV-infected BALB/c and MBT mice on days 0–3 post infection. (b) Percentages of key innate immune cell populations, DCs (CD45+, CD11c+), natural killer (NK) cells (CD45+, CD335+), and neutrophils (CD45+, Ly6C/Ly6G+), after infection with RVFV in the spleen of BALB/c and MBT mice. (c) Linear discriminant (LDA) analyses ranks neutrophils, as having the greatest impact on the dissimilarity between BALB/c and MBT spleen at day 3 post infection. Cytometry experiments were performed twice for each time point (n = 5 mice/strain/timepoint). Representative experiments are displayed. Data are represented as mean ± SEM. Two-way ANOVA was used to test differences between means. *P values < 0.05, **P < 0.01, and ***P < 0.001.
