Figure 8
From: Descemet’s Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits

Whole-mount staining of ZO-1 and α-SMA on a DM stripping cornea. ZO-1 expression in the endothelial cells close to the wound border decreased on day 7, cells on the stromal surface of the wound area were not labeled with CFDA SE but were highly expressed with α-SMA. On day 14, ZO-1 expression was regained in the endothelial cells close to the wound border. CFDA SE negative cells close to the wound border also showed positive ZO-1 expression. α-SMA was maintained in the CFDA SE negative cells, while it was negative in the CFDA SE-labeled cells. Bars represent 20 μm.