Figure 1 | Scientific Reports

Figure 1

From: Vitamin D attenuates myofibroblast differentiation and extracellular matrix accumulation in nasal polyp-derived fibroblasts through smad2/3 signaling pathway

Figure 1

Inhibition of TGF-β1-induced α-SMA mRNA and protein expression by 1,25(OH)2D3 in nasal polyp-derived fibroblasts. Nasal polyp-derived fibroblasts were treated with TGF-β1 and/or 1,25(OH)2D3 or 1,25(OH)2D3 alone for 72 hours. (A,B) Expression levels of α-SMA mRNA and protein were determined by semi-quantitative RT-PCR analysis and Western blotting analysis. Expression of housekeeping GAPDH was used as an internal control. A representative experiment and quantitative determination of α-SMA mRNA and protein levels are shown. (C) Representative fluorescein immunocytochemistry for α-SMA (red) with nuclear DAPI (blue). Scale bar = 50 μm. All data are presented as mean ± SEM. Four primary cell lines from different donors were used. All experiments were performed in at least triplicate and repeated at least three times. *p < 0.05 vs. control, †p < 0.05, ††p < 0.01 vs. TGF-β1.

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