Figure 4 | Scientific Reports

Figure 4

From: Vitamin D attenuates myofibroblast differentiation and extracellular matrix accumulation in nasal polyp-derived fibroblasts through smad2/3 signaling pathway

Figure 4

1,25(OH)2D3 decreases TGF-β1/Smad signaling pathway via reducing Histone 3 acetylation in nasal polyp-derived fibroblasts. Nasal polyp-derived fibroblasts were treated with TGF-β1 and/or 1,25(OH)2D3 or 1,25(OH)2D3 alone or 4 hours. (A) Expression levels of acetylated-histone 3 were determined by Western blotting. Expression of histone 3 was utilized as the internal control. A representative experiment and quantitative determination of protein levels are shown. (B) The mRNA of COL1A1, COL1A2 and α-SMA promoter and its adjacent regions precipitated by acetyl-H3 were quantified by ChIP assay. All data are presented as mean ± SEM. Four primary cell lines from different donors were used. All experiments were performed in at least triplicate and repeated at least three times. *p < 0.05 vs. control, †p < 0.01 vs. TGF-β1.

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