Figure 6 | Scientific Reports

Figure 6

From: Vitamin D attenuates myofibroblast differentiation and extracellular matrix accumulation in nasal polyp-derived fibroblasts through smad2/3 signaling pathway

Figure 6

1,25(OH)2D3 decreases TGF-β1-induced collagen production in ex vivo organ culture of nasal polyps. Nasal polyps were stimulated with TGF-β1 and/or 1,25(OH)2D3 for 72 hours. (A,B) Expression levels of α-SMA and fibronectin mRNA and protein were determined by semi-quantitative RT-PCR analysis and Western blot analysis. Expression of housekeeping GAPDH was utilized as an internal control. A representative experiment and quantitative determination of α-SMA mRNA and protein levels are shown. (C) The amount of total soluble collagen in culture media was quantified using the Sircol assay. All data are presented as mean ± SEM. Four nasal polyp specimens from different donors were used. All experiments were performed in at least triplicate and repeated at least three times. *p < 0.05 vs. control, †p < 0.01 vs. TGF-β1.

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