Figure 4 | Scientific Reports

Figure 4

From: Promysalin is a salicylate-containing antimicrobial with a cell-membrane-disrupting mechanism of action on Gram-positive bacteria

Figure 4

Membrane damage in P. aeruginosa ATCC 10145 and S. thermophilus DSM 20617 T exposed to surfactin. Flow cytometry density diagrams show the SYBR Green I vs PI fluorescence of cells exposed to promysalin or chlorhexidine or both the molecules. Viable cells are gated in G1, viable cells with slightly damaged cell membrane are gated in G2. Dead cells with damaged membrane are gated in G3. The transition of cell population from gate G1 to gate G3 is related to the entity of cell membrane damage. (a) S. thermophilus incubated 60 min at 37 °C in presence of DMSO. (b) S. thermophilus incubated 60 min at 37 °C in presence of surfactin (200 µg/ml). (C) P. aeruginosa cells incubated 60 min at 37 °C in presence of DMSO. (d) P. aeruginosa cells incubated 60 min at 37 °C in presence of surfactin (200 µg/ml). DMSO was added to cell suspension in the same amount which was present in the surfactin solution.

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